妊娠早期蒙古绵羊子宫内膜enJSRV及IFN-τ基因表达水平的分析

本研究旨在检测内源性绵羊肺腺瘤反转录病毒(enJSRV)与干扰素-τ(IFN-τ)在妊娠早期蒙古绵羊子宫内膜组织的表达以及enJSRV的表达与外周血孕酮水平变化的关系。运用TaqMan实时荧光定量PCR技术和电化学发光法对enJSRV和IFN-τ在妊娠早期蒙古绵羊子宫内膜组织相对表达及孕酮水平进行了测定。实时荧光定量PCR结果显示,enJSRV和IFN-τmRNA在妊娠早期绵羊子宫内膜组织有不同程度的表达。SAS统计学软件分析得出,子宫内膜组织中enJSRV mRNA在妊娠12～14d(交配日为0d)表达较高,2～10、16～30d的表达均低于前者。IFN-τmRNA仅在妊娠12～25d表达,14d达其峰值,30d就不能检出,且差异都极显著(P<0.01)。电化学发光法结果显示,孕酮水平在妊娠2d为0.4ng·mL-1,以后升高,妊娠8～16d维持在8.3ng·mL-1左右,在妊娠19～30d孕酮水平有所下降,30d为2.5ng·mL-1。以上结果提示,子宫内膜组织中enJSRV mRNA的表达与外周血孕酮含量及IFN-τ的变化高度相关,且enJSRV在胎盘的形态发生及生殖生物学方面发挥重要作用。     

Characterization of mobile LiDAR data collected with multiple echoes per pulse from crowns during foliation

Abstract

As a state-of-the-art technology for fine-scale forest investigation, mobile terrestrial light detection and ranging (LiDAR), also referred to as mobile laser scanning (MLS), recently has been increasingly exploited to improve its performance on this task. One potential solution is to apply special MLS systems with the capability of collecting multiple echoes per pulse (multi-echoes, for short) from canopies. The methodologies of this rationale turned out to perform better but still insufficiently for canopy properties retrieval, owing to their common defective premise. That is, the knowledge of the characteristics of MLS scan data comprising multi-echoes, so far, is in shortage, especially when regarding the dynamic process of tree foliation. As a pioneering work for this challenge, this study attempted to comprehensively analyze the characteristics of MLS multi-echoes collected from tree crowns during foliation. Specifically, new stable multi-echoes-related features were deduced under the schematic frame of relative quantification, in both spatial and temporal sense. “Relative” here briefly means the division operation deployed on the attributes of multi-echoes, individually in terms of the number of echoes, echo width and crown volume integrity, between their different return orders. Then, the “relative” schematic was primarily validated for more stably representing crown properties during foliation, based on the real data that was collected by the Sensei MLS system with a maximum of three echoes per pulse. Further, a case of tree species classification was examined using a linear discriminant classifier, and it was testified that the resultant temporal statistical rules of multi-echoes as the reverse clues can enhance the performance of MLS in applications.     

Response of Maize and Cowpea Grown Sequentially to Application of Phosphate Rock in the Humid Tropics

Field trials were conducted at the Institute of Agricultural Research and Training Ibadan in 2000 and 2001 cropping seasons to evaluate the efficiency of some phosphorus (P) sources, rates, and frequency of application on grain yields of maize and cowpea grown sequentially. The experiment was laid out in a split–split plot arrangement using randomized complete block design, with three replications. The main plot was frequency of fertilizer application (seasonal and annual). Source of P [single superphosphate (SSP: 18% P₂O₅)]; Ogun rock phosphate (OPR: 20.21% P₂O₅), and crystallizer super (CS: 31.4% P₂O₅) constituted the subplots. The P rates (0, 20, 40, and 60 kg P ha^?1) were the sub-subplots. An early maize cropping followed by a late-season cowpea was the sequential cropping, while an early maize followed by a late-season maize was the continuous cropping. The croppings were repeated the second year. Frequency of P application and source of P were significantly different while rates of application were not. Maize and cowpea grain yields from the control treatment were significantly less than yields from P-treated plots. Maize grain yields were slightly greater in plots alternated with cowpea, compared to continuous maize. Relative agronomic efficiency (RAE) values of OPR and CS were 67% and 75%, respectively, in annual application and 140% and 134% in seasonal applications, respectively, in 2000. In 2001, the trend was almost similar to 2000 except that there was a decline in RAE values of cowpea. Each Naira invested in the annual application returned about ?3.12 while about ?2.13 was returned with the seasonal application (?125.00 is the equivalent of USD$1.00). The OPR and CS can serve as alternatives to water-soluble sources such as SSP.     

Phosphorus Response and Fertilizer Recommendations for Wheat Grown on Nitisols in the Central Ethiopian Highlands

The provision of farmers with proper and balanced fertilizer recommendations is becoming increasingly important, for reasons of crop productivity, food security, and sustainability. Phosphorus (P) response trials with wheat were conducted on Nitisols at 14 sites in the central Ethiopian highlands during the 2010 and 2011 cropping seasons. The treatments, comprising six levels of P fertilizer (0, 10, 20, 30, 40, and 50 kg P ha^?1), were arranged in a randomized complete block design with three replicates. Based on a yield difference between the control and the P treatments, 90% of sites responded to P fertilizer. Application of P fertilizer increased wheat grain yield, up to 30% more than the control. Extractable soil P concentrations (Bray 2, 0–15 cm deep) 3 weeks after planting significantly responded to P fertilizer rate. The critical P concentration (for 90% relative yield) was 13.5 mg kg^?1. Most sites tested had Bray 2 P values <10 mg kg^?1. In the absence of a soil test, a recommendation of 40 kg P ha^?1, resulting in the best response overall, could be made for the first year of application. We also recommend that to prevent a potential loss of wheat yield, a maintenance application of at least 5–12 kg P ha^?1 be applied every year, irrespective of the calculated recommended rate, in order to replace P exported from the field in produce. Further field trials are required to determine interactions between P response and the effects of climate, soil properties, and other management practices.     

高效凝胶过滤色谱法测定肽的相对分子量分布

采用高效凝胶过滤色谱法,对肽粉、酵母核苷酸和自溶酵母粉三种肽类产品的相对分子量大小及分布进行了测定。结果表明,此三种产品的相对分子量分布范围集中在2000Da以下,寡肽含量丰富,其中含有一定量的游离氨基酸和氨基酸残基。该方法简便快捷、准确性高、重复性好,适合肽类产品分子量(<1 0KD)分布的分析检测。     

水蒸气量变化过程中木质室内装饰材料对环境湿度的调节(英文)

为了弄清楚在水蒸气流入流出过程中木质室内装饰材料对环境湿度的调节规律，定量评价室内装饰材料的环境湿度调节性能，该研究在环境恒定温度为２６ ℃条件下，测定了在水蒸气流入流出过程中木质及非木质材料装饰的试验体内环境湿度的经时变化规律，在此基础上定义了ｘ 和ｙ 两个参数来表征材料对环境湿度的调节性能。研究结果表明：（１） 用ｘ 和ｙ 值能够将各种材料按其调湿性能分成４ 个组别。（２） 木质材料类具有较好的环境湿度调节性能，即这些材料能够缓和室内环境湿度的急骤变化。（３） 木质材料的ｘ 和ｙ 值随气积比 Ａ／ Ｖ 的增大呈曲线下降趋势。（４） 在本实验设定的循环过程中，材料厚度对其调湿性能影响不显著。     

Effect of Soil Moisture on Relative Leaf Water Content, Chlorophyll, Proline and Sugar Accumulation in Wheat

The responses of four wheat ( Triticum aestivum L.) varieties to soil moisture were determined by measuring plant parameters such as relative leaf water content (RLWC) and the chlorophyll, proline and sugar contents of the leaf over two seasons. Two levels of irrigation treatments were adopted: irrigated and rainfed. The rainfed plants had consistently higher amounts of proline and sugar but lower RLWC and chlorophyll content. As drought intensified, differences between the irrigated and the rainfed plants with respect to all of these characters increased. The RLWC values of the irrigated plants were significantly higher in the morning but lower values were found at noon, showing some recovery in the afternoon.     

新乡市2016年麦收期2次强对流天气对比分析

为了研究麦收期间强对流天气的发生发展规律，利用常规观测资料、中尺度分析产品和卫星雷达资料，对新乡市2016年麦收期2次强对流过程进行对比分析。结果表明：6月4日的强对流是由地面辐合线触发的；6月5日的强对流是地面冷空气触发的；上干冷下暖湿的垂直结构是产生强对流天气的有利条件；三体散射和悬垂结构预示有冰雹和雷雨大风；中尺度辐合线、辐合区、逆风区的出现和维持是产生局地强降水的一个比较有用的预报依据；维持较大的垂直液态含水量可产生比较大的降水。由此提炼出不同类型强对流天气的预报预警指标，为农业防灾减灾提供科学参考，最大限度地减轻气象灾害造成的不利影响。     

一种宗地四至自动赋值的方法探究

农村土地经营权确权项目关乎民生,项目中需要确权的农村土地不仅数据量大,而且形状各异。其中,宗地四至的信息录入,人工识别效率低且容易出错,若利用现有的地理信息软件工具进行二次开发,实现宗地四至自动赋值,将有效提高效率、降低出错率。笔者探讨了基于ArcGIS Engine的二次开发,用最长界址线法来自动获取宗地四至信息,该方法已经运用到湖南省常德市桃源县农村承包经营权确权登记项目中,达到了一定的效果。     

辽宁地区玉米耗水量与产量的关系

为探讨生长季自然降水条件下水分状况对玉米产量的影响,利用锦州2011、2012年和2014年玉米分期播种试验数据和1981—2010年辽宁农业气象历史资料,研究干旱年份水分与玉米产量的定量关系。结果表明:玉米产量和总耗水量间呈二次抛物线的关系(R~2=0.793,F=105,P=0.0001,n=57),当耗水量达到400~600 mm时,玉米能够获得高产;玉米不同生育阶段需水量呈现抽雄~乳熟期拔节~抽雄期出苗~拔节期乳熟~成熟期播种~出苗期的变化规律,各阶段日耗水强度分别为5.75、4.78、2.36、1.84 mm·d~(-1)和1.35 mm·d~(-1)。在分析相对叶面积指数和相对作物系数关系的基础上,提出了作物水分胁迫系数的相对叶面积订正方法,建立了以Jenson模型为基础的玉米水分生产函数模型。